Confirming yeast two-hybrid protein interactions using in vitro glutathione-S-transferase pulldowns.
نویسندگان
چکیده
1 The Two-Hybrid System: A Personal View Stanley Fields and Paul L. Bartel .............................................. 3 2 Growth and Maintenance of Yeast Lawrence W. Bergman ................................................................ 9 3 Media Formulations for Various Two-Hybrid Systems Michael Saghbini, Denise Hoekstra, and Jim Gautsch ........ 15 4 Yeast Two-Hybrid Vectors and Strains Philip James ............................................................................... 41 5 High-Efficiency Transformation of Plasmid DNA into Yeast Robin A. Woods and R. Daniel Gietz ...................................... 85 6 Qualitative and Quantitative Assessment of Interactions Monica M. Montano ................................................................... 99 7 Strategies for Rescuing Plasmid DNA from Yeast Two-Hybrid Colonies Alyson Byrd and René St-Arnaud ......................................... 107
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ورودعنوان ژورنال:
- Methods in molecular biology
دوره 177 شماره
صفحات -
تاریخ انتشار 2001